HiBiT Protein Tagging System
Detect and quantify any tagged protein with a simple, luminescent signal.
HiBiT simplifies protein detection, providing a streamlined, antibody-free protocol for detecting tagged proteins using a convenient, bioluminescence based method. With the dynamic range to detect proteins without overexpression, the convenience of a single-reagent-addition method, and live cell detection options, HiBiT technology opens up a world of possibilities for researchers studying protein biology.
Sensitive quantification of HiBiT-tagged proteins in cell lysates, IP complexes, or other cell-free systems.
Used with live cells to quantify surface-expressed or secreted proteins.
Detects any tagged protein on a blot in a few minutes with a simple luminescent signal.
Monitor Receptor Internalization
The HiBiT detection method eliminates the need for antibody-based methods for receptor internalization studies. With HiBiT technology, the multiple antibody binding steps and associated washes are eliminated from detection protocols. Simply add the detection reagent and measure a luminescent signal.
Quantify Protein Abundance and Degradation
Classic epitope tagging methods are limited in throughput or sensitivity, require high-quality antibodies and may only yield semi-quantitative results. HiBiT tagging brings the simplicity and sensitivity of bioluminescence to studies on protein abundance, quantifying proteins down to endogenous levels, even those maintained at low expression levels.