Two-Step Reverse Transcription and qPCR
The GoTaq? 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using GoScript? Reverse Transcription System and GoTaq? qPCR Master Mix.
The GoTaq? qPCR Master Mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq? Hot Start Polymerase, MgCl2, dNTPs, BRYT Green? Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.
The GoScript? Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.